alamarBlue^® 阿爾瑪藍(lán)（細(xì)胞增殖及毒性檢測(cè)試劑）

基本描述：

阿爾瑪藍(lán)（Alamar Blue）是一種細(xì)胞增殖檢測(cè)試劑，對(duì)各種人和哺乳動(dòng)物細(xì)胞、細(xì)菌和真菌提供一種快速和敏感的細(xì)胞增殖和毒性檢測(cè)方法。

阿爾瑪藍(lán)（Alamar Blue）是一種基于刃天青（resazurin）的檢測(cè)試劑。刃天青作為一種一種氧化還原（REDOX）指示劑，根據(jù)細(xì)胞代謝還原情況發(fā)生顏色變化。氧化態(tài)的刃天青呈紫藍(lán)色且基本無(wú)熒光，其還原態(tài)產(chǎn)物試鹵靈（resorufin）轉(zhuǎn)變?yōu)榉奂t色且高度熒光，產(chǎn)生的熒光強(qiáng)度與呼吸活細(xì)胞數(shù)量呈正比。通過(guò)檢測(cè)呼吸過(guò)程中氧化水平，阿爾瑪藍(lán)用作一種定量檢測(cè)細(xì)胞活力和毒性的直接指示劑。阿爾瑪藍(lán)的顏色變化可通過(guò)普通分光光度計(jì)檢測(cè)吸光度變化，檢測(cè)波長(zhǎng)為570nm，參考波長(zhǎng)為600nm；阿爾瑪藍(lán)的熒光變化可通過(guò)熒光光度計(jì)檢測(cè)，激發(fā)波長(zhǎng)在530~560nm之間，發(fā)射波長(zhǎng)為590nm。

檢測(cè)原理：（細(xì)胞增殖實(shí)驗(yàn) Cell proliferation assay））

※ 生長(zhǎng)中細(xì)胞引起Alamar Blue的化學(xué)還原反應(yīng)；

※ 持續(xù)性細(xì)胞生長(zhǎng)維持還原環(huán)境，使得Alamar Blue呈紅色，發(fā)強(qiáng)熒光；

※ 生長(zhǎng)受抑制維持氧化環(huán)境，使得Alamar Blue呈藍(lán)色，無(wú)熒光；

※ 使用基于熒光或吸光值檢測(cè)的儀器收集數(shù)據(jù)；

※ 熒光檢測(cè)的激發(fā)波長(zhǎng)為530~560nm之間，發(fā)射波長(zhǎng)為590nm；

※ 吸光值檢測(cè)的波長(zhǎng)為570nm，參考波長(zhǎng)為600nm；

保存方法：

室溫12個(gè)月穩(wěn)定，2-8°C保存20個(gè)月穩(wěn)定；避光保存；

產(chǎn)品優(yōu)勢(shì)：

• 簡(jiǎn)單：水溶性，只需添加和測(cè)量即可；
• 靈活：顯色和熒光檢測(cè)，懸浮細(xì)胞和貼壁細(xì)胞；
• 無(wú)毒：對(duì)細(xì)胞無(wú)毒，對(duì)使用者和環(huán)境也無(wú)毒；
• 可靠：大量引用用于細(xì)胞毒性和活力檢測(cè)；
• 規(guī)模化：簡(jiǎn)單放大體系用于高通量分析
• 高靈敏：zui低可檢測(cè)到50個(gè)細(xì)胞
• 穩(wěn)定高：*緩沖配方使其能用于長(zhǎng)期研究；
• 經(jīng)濟(jì)：不需要細(xì)胞裂解，使細(xì)胞能繼續(xù)培養(yǎng)用于后續(xù)分析；

訂購(gòu)信息：品牌保證，現(xiàn)貨供應(yīng)，大量使用文獻(xiàn)，國(guó)內(nèi)外上萬(wàn)實(shí)驗(yàn)室的認(rèn)可品質(zhì)，咨詢，，：。

【注1】：按照96孔板，每孔100µl終體積來(lái)算，5ml alamarBlue^®可做500個(gè)孔；25ml可做2500個(gè)孔。

【注2】：alamarBlue^® Technical Datasheet

引用文獻(xiàn)（AbdSerotech alamarBlue^®，43篇）：

1. Lewis, C.S. et al. (2010) Local Antibiotic Delivery with Bovine Cancellous Chips.
2. Alsford, S. and Horn, D. (2011) Elongator Protein 3b Negatively Regulates Ribosomal DNA Transcription in African Trypanosomes.
3.  Crilly, A. et al. (2011) Phosphodiesterase 4 (PDE4) regulation of proinflammatory cytokine and chemokine release from rheumatoid synovial membrane.
4. Paget, C. et al. (2011) Potential Role of Invariant NKT Cells in the Control of Pulmonary Inflammation and CD8+ T Cell Response during Acute Influenza A Virus H3N2 Pneumonia.
5. Lakhkar, N. et al. (2011) Titanium and strontium-doped phosphate glasses as vehicles for strontium ion delivery to cells.
6. Wilson, B.A. et al. (2011) High-throughput screen identifies novel inhibitors of cancer biomarker a-methylacyl coenzyme A racemase (AMACR/P504S).
7. Lau, L.I. et al. (2011) The Effect of Photooxidative Stress and Inflammatory Cytokine on Complement Factor H Expression in Retinal Pigment Epithelial Cells.
8. Arlian, B.M. and Tinker, J.K. (2011) Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A2/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice.
9. Voloshin, T. et al. (2011) G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist.
10. Rao, T.D. et al. (2011) Dual-Fluorescence Isogenic High-Content Screening for MUC16/CA125 Selective Agents.
11. Uitdehaag, J.C. et al. (2011) Multidimensional Profiling of CSF1R Screening Hits and Inhibitors: Assessing Cellular Activity, Target Residence Time, and Selectivity in a Higher Throughput Way.
12. Rzhepishevska, O. et al (2011) The antibacterial activity of ga3+ is influenced by ligand complexation as well as the bacterial carbon source.
13. Xu, S. et al. (2011) Marek's disease virus type 1 microRNA miR-M3 suppresses cisplatin-induced apoptosis by targeting Smad2 of the transforming growth factor beta signal pathway.
14.  Ardakani, A.G. et al. (2014) Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.
15. Nakayama, G.R. et al. (1997) Assessment of the Alamar Blue assay for cellular growth and viability in vitro.
16. Diril, M.K. et al. (2012) Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration.
17.  Warrier, T. et al. (2012) Antigen 85C inhibition restricts Mycobacterium tuberculosis growth through disruption of cord factor biosynthesis.
18. Dreidax, D. et al. (2013) Low p14ARF expression in neuroblastoma cells is associated with repressed histone mark status, and enforced expression induces growth arrest and apoptosis.
19. Wang, H. et al. (2014) Enhanced osteoblast responses to poly ether ether ketone surface modified by water plasma immersion ion implantation.
20. Park, K.H. et al. (2014) Expression of polysialylated neural cell adhesion molecules on adult stem cells after neuronal differentiation of inner ear spiral ganglion neurons.

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